Quiz on Biotechnology and Genetic Engineering

1. Two bacteria found to be very useful in genetic engineering experiments are
Escherichia and Agrobacterium
Nitrobacter and Azotobacter
Nitrosomas and Klebsiella
Rhizobium and Diplococcus

2. Crown gall disease in plants is caused by
Ti plamid
Pi plasmid

3. Golden rice is a promising transgenic crop. When released for cultivation, it will help in
pest resistance
alleviation of vitamin A deficiency
high lysine content
insect resistance

4. Gel electrophoresis is used for
isolation of DNA molecules
cutting of DNA into fragments
separation of DNA fragments according to their size
construction of recombinant DNA by joining with cloning vectors

5. Which of these are most widely used in genetic engineering?

6. New strain of bacteria which is used in chewing up oil spoilage is
Escherichia coli
Bacillus subtilis
Salmonella typhimurium
Pseudomonas putida

7. Cryopreservation of seeds is done at:
0 oC
-10 oC
-196 oC
-25 oC

8. Electroporation involves
promotion of seed germination by induced imbibition of water with electric current
passage of sucrose through sieve pores by electroosmosis
purification of saline water with the help of an artificial membrane
making transient pores in cell membrane to facilitate entry of gene constructs

9. Known sequence of DNA that is used to find complementary DNA strands, is
DNA probe
recombinant DNA

10. Variable Number of Tandem Repeats (VNTRs) in the DNA molecule are highly useful in
DNA finger printing
stem cell culture
recombinant DNA technology
monoclonal antibody production

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