Quiz on Biotechnology Principles and Processes

This quiz is designed to assess your basic knowledge in 'biotechnology'.  Choose the best answer from the four options given. When you've finished answering as many of the questions as you can, scroll down to the bottom of the page and check your answers by clicking 'Score'.  Percentage scores will be displayed along with the right answers. 

1. The bacteria generally used for genetic engineering is:
Agrobacterium
Bacillus
Pseudomonas
Clostridium

2. In gel electrophoresis, separated DNA fragments can be visualized with the help of
acetocarmine in UV radiation
ethidium bromide in infrared radiation
acetocarmine in bright blue light
ethidium bromide in UV radiation

3. Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? :
λ−phage
Ti plasmid
Retrovirus
pBR322

4.Biolistics (Gene-gun) is suitable for
DNA fingerprinting
Disarming pathogen vectors
Constructing rDNA by joining with vectors
Transformation of plant cells

5.Restriction endonuclease
Tare present in mammalian cells for degradation of DNA when the cell dies
are used in genetic engineering for ligating two DNA molecules
are used tor in vitro DNA synthesis
are synthesised by bacteria as part of their defense mechanism

6. Which of the following enzymes are used to join bits of DNA
Ligase
Primase
DNA polymerase
Endonunuclease

7.Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
Extension, Denaturation, Annealing
Annealing, Denaturation, Extension
Denaturation, Extension, Annealing
Denaturation, Annealing, Extension

8.Which one of the following equipment is essentially required for growing microbes on a large scale for industrial production of enzymes?
BOD incubator
Sludge digester
Bioreactor
Industrial oven

9.Stirred-tank bioreactors have been designed for
Availability of oxygen throughout the process
Purification of the product
Ensuring anaerobic conditions in the culture vessel
Addition of preservatives to the product

10. Which of the following is not correctly matched for the organism and its cell wall degrading enzyme??
Bacteria–Lysozyme
Plant cells–Cellulase
Fungi–Chitinase
Algae-Methylase

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