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Multiple choice Quiz on DNA Sequencing

This quiz is designed to assess your  knowledge in ‘DNA sequencing’. Choose the best answer from the four options given. When you've finished answering as many of the questions as you can, scroll down to the bottom of the page and check your answers by clicking 'Score'. Percentage score will be displayed along with right answers.
1. Which of the following is a chemical sequencing method?
DNA chip sequencing
Edman's method
Sanger’s method
Maxam-Gilbert method
2. Which of the following is called as dideoxynucleotide chain termination method?
Sanger's method
Maxam-Gilbert method
Edman's method
Automated sequencing method
3. How many ddNTPs are used in Sanger’s method?
1
2
3
4
4. In automated DNA sequencing,
radio labelled dNTPs are used
radio labelled ddNTPs are used
fluorescently labelled dNTPs are used
fluorescently labelled ddNTPs are used
5. In Maxam-Gilbert method, chemical used for cytosine alteration is
formic acid
hydrazine
Dimethyl sulphate
piperidine
6. The chemical used for strand cleavage in Maxam-Gilbert method is
Piperidine
hydrazine
formic acid
DMSO
7. ddNTP is different from dNTP in having
H in place of OH in 3 position of dNTP
OH in place of H in 3 position of dNTP
OH in place of H in 2 position of dNTP
CH3 in place of OH in 3 position of dNTP
8. Sanger sequenced the first complete genome of
T4 phage
C. elegans
TMV
ФX 174
9. Automated DNA sequencing is an improvement of Sanger’s method where
ddNTPS are used for chain termination
Fluorescently labelled dNTPs are used for chain termination
Fluorescently labelled ddNTPs are used for chain termination
PCR is used for making sequencing templates
10. Frederick Sanger won Nobel Prize in 1980 for sequencing phage ФX 174. He was already awarded with Nobel Prize in 1958 for
discovery of Sanger’s method
elucidating amino acid sequence of insulin
elucidating nucleotide sequence of insulin
elucidating nucleotide sequence of somatostatin
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