Diagram Quiz on Steps in recombinant DNA or rDNA technology

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Steps in rDNA technology Diagram
1. The first step in rDNA technology, labelled 1 is
isolation of gene of interest
Identification and isolation of donor gene
Selection of donor gene

2. It is double stranded, self replicating, circular DNA molecule present in bacteria which is widely used as a gene cloning vector. The structure labelled 2 in the figure is
bacterial genome

3. These enzymes are called as molecular scissors which is essential for making internal cuts in a DNA molecule or vector at specific sites. The enzyme used in making cut in the vector (labelled 3) is
restriction endonuclease
restriction exonuclease
polynucleotide kinase

4. The cut by the enzyme in the vector (labelled 4) creates single stranded unpaired regions of DNA. This type of cut pattern is called as
even cut
loose cut
staggered cut
blunt end

5. 5'GAATTC3' is a restriction site of a widely used restriction enzyme which produces sticky ends. The enzyme is
Bam H1
Hind III
Eco R1
Hind II

6. In the figure labelled 6, the DNA strand is the
gene of interest with sticky end
gene of interest with blunt end
chimeric DNA

7. This enzyme is called as molecular glue which is used to join two DNA strands by forming phosphodiester bond. The joining enzyme labelled 7 is
restriction enzyme type I
terminal transferase

8. The vector (plasmid) with foreign gene inserted is called (labelled 8)
recombinant DNA
chimeric DNA
cp DNA
both a and b

9. The figure labelled 9 is the process of introducing recombinant vector into a suitable host like bacterium.The process is called

10. In the figure, 10 a and 10 b are processes that lead to the formation of protein product encoded by the gene of interest. 10 a and 10 b are
replication and transcription
transcription and translation
transcription and transformation
replication and transformation

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