Multiple Choice Quiz on Molecular Biology Techniques | Biotechnology Quiz

This quiz is designed to assess your basic knowledge in ‘molecular biology techniques’. Choose the best answer from the four options given. When you've finished answering as many of the questions as you can, scroll down to the bottom of the page and check your answers by clicking 'Score'. Percentage scores will be displayed along with the right answers.

1. The polymerase chain reaction or PCR is a technique that
was used to demonstrate DNA as the genetic material
is used to determine the content of minera
uses short DNA primers and a thermostable DNA polymerase to replicate specific DNA sequences in vitro
measures the ribosome transfer rate during translation

2. Positional cloning refers to:
using a selection procedure to clone a cDNA
cloning a portion of a gene using PCR
Isolating a gene by PCR using primers from another species
Mapping a gene to a chromosomal region and then identifying and cloning a genomic copy of the gene from the region

3. RFLP analysis is a technique that
uses hybridization to detect specific DNA restriction fragments in genomic DNA
Is used to determine whether a gene is transcribed in specific cells
Measures the transfer frequency of genes during conjugation
Is used to detect genetic variation at the protein level

4. The PCR used to generate single-stranded copies of a DNA sequencing or can be used as probes or even as primers
Inverse PCR
RT PCR
Real Time PCR
Asymmetric PCR

5. Which of the following is the function of polynucleotide kinase, the enzyme used in DNA recombinant technology?

Add a phosphate to the 3' OH end of the polynucleotide to label it or permit ligation
Add phosphate tails to the 3' OH ends of a linear duplex
Add phosphate tails to the 5' OH ends of linear duplex
None of the above

6. Techniques used for the study of gene expression
DNA microarrays
DNA hybridisation
Both
None of these

7. The techniques of transfer of DNA molecules separated by gel electrophoresis to the nitrocellulose or nylon membrane is called
Northern Blot
Southern Blot
Western Blot
None of these

8. DNA sequencing is done by
Maxam Gilbert
Sanger dideoxy method
Watson -Crick
Maxam Gibert and Sanger dideoxy method

9. The length of the DNA associated with a protein is determined using the technique
DNA replication
DNA fingerprinting
DNA printing
DNA footprinting

10. Small samples of proteins are detected by
Southern Blotting
Northern Blotting
Eastern Blotting
Western Blotting
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